Published: September 25th, 2015
Revised: June 7th, 2016
Quantitative real-time PCR (qPCR) DNA-based method specifically for testing Legionella pneumophila in water samples that has the advantage of much greater sensitivity coupled with the potential to provide results in as little as 24 hrs after submission to the lab. The method involves DNA extraction and DNA testing by quantitative polymerase chain reaction (qPCR). This method has been extensively validated, and has been used widely in Europe for several years. While this test cannot differentiate between active colonization and residual DNA from dead cells, its strength is as a rapid, highly conservative screen for this important pathogen to guide urgent public health decision-making. When rapid turn-around-time and definitive, accurate detection and precise quantification are required, our Legionella pneumophila quantitative (real-time) PCR DNA-based test is the recommend choice.
When to choose qPCR Legionella pneumophila water test
QPCR rush results can be available within 24 hrs or less. Our regular turn-around-time is typically 48 hrs. Most methods for testing fluids for L. pneumophila rely on culture. Although culture-based methods remain the current gold-standard means of determining the presence of this bacterium, this technique has poor sensitivity (negative results cannot reliably predict the absence of contamination), and they typically require 10-14 days to complete testing. This method has been extensively validated, and has been used widely in Europe for many years; it was approved in 2006 by the French Association of Normalization (AFNOR), a parallel standards body to the Canadian Standards Association (CSA). Since 2012, Public Health Ontario changed to a qPCR test for Legionella for lower respiratory tract specimens.
New guideline values are provided in Public Works and Government Service’s MD 15161 – 2013 ”Control of Legionella in Mechanical Systems Standard for Building Owners, Design Professionals, and Maintenance Personnel, Addendum C”, published in March 2016. These guideline values apply to Normal Operation Mode only, for Emergencies and /or outbreaks of disease, additional assessment and measures are required. As illustrated in the table below test levels are the same for all water sources, however, recommended actions may differ depending of the water source.
Sample collection procedure
- Collect water (a 500 mL bottle with preservative is provided by Sporometrics) in sterile, screw-top bottles. For water sources expected to contain disinfectant chemicals such as chlorine, the collection container should include a suitable preservation buffer (the US-CDC recommends sodium thiosulfate to a final concentration of 0.1 M). Sporometrics provides sample collection containers to meet your needs. Please contact us prior to sampling to make arrangements.
- Collect culture swabs of internal surfaces of faucets, aerators, and shower heads in a sterile, screw-top container (e.g., 50 mL plastic centrifuge tube, with or without preservation buffer, according to the above guidance). Submerge each swab in approximately 5 mL of sample water taken from the same device from which the sample was obtained.
- Transport samples to the laboratory as soon as possible after collection. Samples may be transported at room temperature but must be protected from temperature extremes. Samples not processed with 24 hours of collection must be refrigerated.
Published: September 29th, 2014
Revised: September 29th, 2014
In a follow-up to Adams’ WIRED article “The Angel’s Share”, the Distillation chapter of Proof tells the story of how Sporometrics’ Dr. James Scott found himself studying mycology and delves deeper into the mystery of the whiskey fungus he investigated.
Proof expands upon Adam Rogers’ 2011 WIRED magazine article “The Angel’s Share”; the story of Sporometrics’ Dr. James Scott’s discovery of not just a new species, but a completely new genus of fungi, identified on trees, street signs, and buildings surrounding whiskey warehouses in Lakeshore Ontario, then around distilleries across the globe. The unmasking of the whiskey fungus Baudoinia compniacensis is just one of dozens of tales Rogers tells as he uncovers the science of alcohol production, powered by physics, molecular biology, organic chemistry, and a bit of metallurgy-and our taste for the products is a melding of psychology and neurobiology.
Published: March 16th, 2014
Revised: July 21st, 2014
Using cutting edge high through-put DNA sequencing, Sporometrics CEO Dr. James Scott and his colleagues investigated the bacterial make-up of faeces from young babies and the homes where the babies lived.
Scott’s group found a significant overlap in bacterial communities in a baby’s faeces and dust from their home, suggesting that a baby may be sharing their gut bacteria with the environment and vice versa.
This finding may have long-ranging implications on how our environments may influence our lives. How much of a personal imprint do we leave on our home? When we move to a new home, does the microbial imprint of the former occupants have the potential to affect us? And are these effects good or bad? Sorting out these interesting questions will be the focus of Scott’s future research.
Reference: Konya T, Koster B, Maughan H, Escobar M, Azad MB, Guttman DS, Sears MR, Becker AB, Brook JR, Takaro TK, Kozyrskyj AL, Scott JA, and the CHILD Investigators. 2014. Associations between bacterial communities in house dust and infant gut. Environmental Research 131: 25-30. doi: 10.1016/j.envres.2014.02.005.
Published: February 10th, 2014
Revised: February 15th, 2014
Tune in to Daily Planet February 13th, 2014 at 7 PM EDT on Daily Planet to watch James and Lucas testing out Phone Soap, a nifty, high-tech gadget that gets rid of nasty bacteria from your cell phone!
First report on the molecular identification of the phytoplasma associated with a lethal yellowing-type disease of coconut palms in Côte d’Ivoire
Published: August 8th, 2013
Revised: July 21st, 2014
Cocos nucifera is considered the most important crop along the coastal belt of West Africa. Particularly in Côte d’Ivoire, coconut palm is cultivated on approximately 50,000 ha and produces an average of 45,000 tonnes of copra per year, which represents the main source of income for people living in the coastal region. Lethal yellowing (LY)-type diseases affecting coconut and other palm species worldwide have mostly been associated with phytoplasma strains of group 16SrIV ‘Coconut lethal yellows’. However, LY-type diseases like Cape St Paul wilt in Ghana (CSPW), the “maladie de Kaincopé” in Togo and Awka disease (Lethal Decline, LD) in Nigeria, previously included in 16SrIV group have been recently classified as a new group 16SrXXII. LY-type disease has quickly developed among coconut palms in the Grand-Lahou in Côte d’Ivoire, currently affecting more than 7000 hectares. We present the first report on the molecular identification of the phytoplasma associated with a lethal yellowing-type disease of coconut palms in Cote d’Ivoire.