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Environmental Analysis

Heterotrophic plate count, semiquantitative [C221]

Published: July 8th, 2009

Revised: July 5th, 2013

This test measures total culturable bacteria contained in a sample of bulk material by culturing a measured amount of the sample on Tryptic Soy Agar and incubating at 35 °C for 48 hr. At the end of the incubation period, bacterial colonies are counted and reported as 1+, 2+, 3+, where the numeral preceding the “plus sign” indicates the order of magnitude of the number of colonies observed. This test is suitable for bulk samples, swab samples and many other matrices.

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